ZBTB46 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13579R-RBITC |
| Product Name | ZBTB46 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Sheep, Horse, Chicken |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ZBTB46/BTBD4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 140685 |
| Subcellular location | Nucleus |
| Synonyms | BTB/POZ domain containing protein 4; BTBD4; RINZF; Zinc finger and BTB domain containing 46; Zinc finger and BTB domain containing protein 46; Zinc finger protein 340; ZNF340; ZBT46_HUMAN. |
| Background | BTBD4 is a 589 amino acid protein that contains one BTB/POZ domain. The BTB/POZ domain mediates homomeric and heteromeric POZ-POZ interactions and is common to transcriptional regulators involved in chromatin modeling. In several BTB/POZ containing proteins, including BCL-6 and the promyelocytic leukemia zinc-finger (PLZF) oncoprotein, this domain interacts with the SMRT/N-CoR-mSin3A HDAC complex and is directly involved in repressing and silencing gene transcription. When this domain is deleted, as with the oncogenic PLZF-RAR chimera of promyelocytic leukemias, this transcriptional repression is attenuated. This suggests that BTBD4 may play a role in transcription regulation. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is ZBTB46 Polyclonal Antibody, RBITC Conjugated (bs-13579R-RBITC) validated for, and at what dilution?
bs-13579R-RBITC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ZBTB46 Polyclonal Antibody, RBITC Conjugated (bs-13579R-RBITC) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Sheep, Horse, Chicken.
How should bs-13579R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ZBTB46 Polyclonal Antibody, RBITC Conjugated (bs-13579R-RBITC)?
KLH conjugated synthetic peptide derived from human ZBTB46/BTBD4
What are the excitation and emission wavelengths of the RBITC conjugate (bs-13579R-RBITC)?
RBITC on bs-13579R-RBITC: excitation / emission 540nm/625nm.