IRF6 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13592R-Cy3 |
| Product Name | IRF6 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Cow, Sheep, Pig, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human IRF6 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Interferon regulatory factor 6; IRF 6; IRF-6; IRF6; IRF6_HUMAN; LPS; OFC 6; OFC6; OTTHUMP00000034677; OTTHUMP00000034678; PIT; PPS; PPS1; VWS; VWS1. |
| Background | Interferon regulatory factor-1 (IRF-1) and IRF-2 have been identified as novel DNA-binding factors that function as regulators of both type I interferon (interferon-alpha and beta) and interferon-inducible genes. The two factors are structurally related, particularly in their N-terminal regions, which confer DNA binding specificity. In addition, both bind to the same sequence within the promoters of interferon-alpha and interferon-beta genes. IRF-1 functions as an activator of interferon transcription, while IRF-2 binds to the same cis elements and represses IRF-1 action. IRF-1 and IRF-2 have been reported to act in a mutually antagonistic manner in regulating cell growth; overexpression of the repressor IRF-2 leads to cell transformation while concomitant overexpression of IRF-1 causes reversion. IRF-1 and IRF-2 are members of a larger family of DNA binding proteins that includes IRF-3, IRF-4, IRF-5, IRF-6, IRF-7, ISGF-3 gamma p48 and IFN consensus sequence-binding protein (ICSBP). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is IRF6 Polyclonal Antibody, Cy3 Conjugated (bs-13592R-Cy3) validated for, and at what dilution?
bs-13592R-Cy3 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does IRF6 Polyclonal Antibody, Cy3 Conjugated (bs-13592R-Cy3) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow, Sheep, Pig, Rabbit.
How should bs-13592R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise IRF6 Polyclonal Antibody, Cy3 Conjugated (bs-13592R-Cy3)?
KLH conjugated synthetic peptide derived from human IRF6
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-13592R-Cy3)?
Cy3 on bs-13592R-Cy3: excitation / emission 512,550nm/570,615nm.