SPANXB1 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-13618R-BF488 |
| Product Name | SPANXB1 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SPANXB1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Cancer/testis antigen 11.2; Cancer/testis antigen family 11, member 2; CT11.2; hCG2036708; Nuclear associated protein SPAN Xb; Nuclear-associated protein SPAN-Xb; Nuclear-associated protein SPAN-Xf; SPAN Xb protein; SPAN Xf; SPANX; SPANX B; SPANX C; SPANX F; SPANX family member B/F; SPANX family, member B1; SPANX family, member B2; SPANX family, member F1; SPANX-B; SPANX-F; SPANXB; SPANXB2; SPANXF; SPANXF1; Sperm protein associated with the nucleus on the X chromosome B/F; Sperm protein associated with the nucleus, X chromosome, family; Sperm protein associated with the nucleus, X chromosome, family member B1; Sperm protein associated with the nucleus, X chromosome, family member B2; SPNXB_HUMAN. |
| Background | A variety of morphological and molecular changes are required for mature spermatozoa formation. These steps are temporally guided by the transcription and translation of several testis-specific genes. SPANX (sperm protein associated with the nucleus, X-linked) family members are sperm- and testis-specific proteins containing between 97-103 amino acids, whose genes form a cluster on chromosome X. Sharing a high level of sequence similarity, SPANX-A, -B, -C, -D and -E localize to both cytoplasm and nucleus where they are associated with nuclear craters. SPANX-B (Sperm protein associated with the nucleus on the X chromosome B/F) is a 103 amino acid protein that is detected in round and elongating spermatids. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SPANXB1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-13618R-BF488) validated for, and at what dilution?
bs-13618R-BF488 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-13618R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SPANXB1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-13618R-BF488)?
KLH conjugated synthetic peptide derived from human SPANXB1
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-13618R-BF488)?
AbBy Fluor-488 on bs-13618R-BF488: excitation / emission 499nm/519nm.