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CLEC9A Polyclonal Antibody, Cy7 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
Overview
Catalog # bs-13620R-Cy7
Product Name CLEC9A Polyclonal Antibody, Cy7 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CLEC9A
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 283420
Swiss Prot Q6UXN8
Subcellular location Cell membrane
Synonyms C type lectin domain family 9, member A; C-type lectin domain family 9 member A; CLC9A_HUMAN; CLEC9A; DNGR1; HEEE9341; PRO34046; UNQ9341.
Background The C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily consists of a variety of proteins that share a common protein fold and have diverse functions, including cell-cell signaling, cell adhesion, glycoprotein turnover and immune responses. CLEC-9A (C-type lectin domain family 9 member A), also known as DNGR1 (dendritic cell natural killer lectin group receptor 1), is a 241 amino acid single-pass type II membrane protein that contains one C-type lectin domain and belongs to the CTL/CTLD superfamily. Expressed in myeloid lineage cells, brain, spleen and thymus, CLEC-9A is a group V C-type lectin-like receptor (CTLR) that acts as an activation receptor. The gene encoding CLEC-9A maps to human chromosome 12p13.2 and mouse chromosome 6 F3.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CLEC9A Polyclonal Antibody, Cy7 Conjugated (bs-13620R-Cy7) validated for, and at what dilution?

bs-13620R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CLEC9A Polyclonal Antibody, Cy7 Conjugated (bs-13620R-Cy7) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.

How should bs-13620R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CLEC9A Polyclonal Antibody, Cy7 Conjugated (bs-13620R-Cy7)?

KLH conjugated synthetic peptide derived from human CLEC9A

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-13620R-Cy7)?

Cy7 on bs-13620R-Cy7: excitation / emission 743nm/767nm.