AP1M2 Polyclonal Antibody

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-AP1M2 Polyclonal Antibody, Unconjugated (bs-13638R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Mouse small intestine lysates probed with AP1M2 Polyclonal Antibody, unconjugated (bs-13638R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.$

Mouse small intestine lysates probed with AP1M2 Polyclonal Antibody, unconjugated (bs-13638R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.

$Lane 1: Human HepG2 cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human A431 cell lysates probed with AP1M2 Polyclonal Antibody, Unconjugated (bs-13638R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human HepG2 cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human A431 cell lysates probed with AP1M2 Polyclonal Antibody, Unconjugated (bs-13638R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Predicted Reactivity

Mouse
Cow
Sheep
Pig
Horse

Overview
Catalog #	bs-13638R
Product Name	AP1M2 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human
Predicted Reactivity	Mouse, Cow, Sheep, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human AP1M2
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10053
Swiss Prot	Q9Y6Q5
Subcellular location	Cytoplasm, Cell membrane
Synonyms	mu2; MU1B; MU-1B; HSMU1B; AP1-mu2; AP-1 complex subunit mu-2; AP-mu chain family member mu1B; Adaptor protein complex AP-1 subunit mu-2; Adaptor-related protein complex 1 subunit mu-2; Clathrin assembly protein complex 1 mu-2 medium chain 2; Golgi adaptor HA1/AP1 adaptin mu-2 subunit; Mu-adaptin 2; Mu1B-adaptin; AP1M2
Background	Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200