GRB 14 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13641R-BF350 |
| Product Name | GRB 14 Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This antibody may also detect the similar protein GRB10 due to an 83% sequence similarity in the immunogen sequence. |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GRB 14 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2888 |
| Swiss Prot | Q14449 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Growth factor receptor-bound protein 14; GRB14 adapter protein; GRB14 |
| Background | Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GRB 14 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-13641R-BF350) validated for, and at what dilution?
bs-13641R-BF350 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GRB 14 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-13641R-BF350) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Horse, Chicken, Rabbit.
How should bs-13641R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GRB 14 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-13641R-BF350)?
KLH conjugated synthetic peptide derived from human GRB 14
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-13641R-BF350)?
AbBy Fluor-350 on bs-13641R-BF350: excitation / emission 346nm/442nm.