MAP4K5 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13654R-Cy3 |
| Product Name | MAP4K5 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Cow, Horse, Chicken |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MAP4K5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm |
| Synonyms | EC 2.7.11.1; KHS; Kinase homologous to SPS1/STE20; MAPK/ERK kinase kinase kinase 5; MEK kinase kinase 5; MEKKK 5; M4K5_HUMAN. |
| Background | Several mammalian kinases have been identified with sequence similarity to the Saccharomyces cerevisiae serine/threonine kinase STE20. STE20 is involved in relaying signals from G-protein coupled receptors to cytosolic MAP kinase cascades, and it lies upstream of a MAP kinase kinase kinase. Mammalian STE20-like kinases include KHS, GLK, NIK, YSK1, HPK1, Krs-1, Krs-2 and GC kinase. KHS (for kinase homologous to SPS1/STE20) is a protein that is most closely related to GC kinase. The KHS kinase has been shown to activate a variety of substrates, including JNK, suggesting a role in stress response. |
| Application Dilution | |
| IF(IHC-P) | WB1:300-5000 |
| IF(IHC-F) | IFIHC-P1:50-200 |
| IF(ICC) | IFIHC-F1:50-200 |
Frequently asked questions
Which applications is MAP4K5 Polyclonal Antibody, Cy3 Conjugated (bs-13654R-Cy3) validated for, and at what dilution?
bs-13654R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MAP4K5 Polyclonal Antibody, Cy3 Conjugated (bs-13654R-Cy3) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Horse, Chicken.
How should bs-13654R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MAP4K5 Polyclonal Antibody, Cy3 Conjugated (bs-13654R-Cy3)?
KLH conjugated synthetic peptide derived from human MAP4K5
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-13654R-Cy3)?
Cy3 on bs-13654R-Cy3: excitation / emission 512,550nm/570,615nm.