MKLN1 Polyclonal Antibody
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-13655R |
| Product Name | MKLN1 Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Sheep |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MKLN1 |
| Immunogen Range | 301-400/735 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm |
| Synonyms | MKLN 1; Mkln1; MKLN1_HUMAN; muskelin 1, intracellular mediator containing kelch mots; Muskelin. |
| Background | Muskelin is a 735 amino acid nuclear and cytoplasmic protein that mediates cytoskeletal responses and cell spreading to Thrombospondin 1, an extracellular matrix glycoprotein. Forming a CTLH complex with RMND5A, Ran BP-M and TWA1, Muskelin exists as two alternatively spliced isoforms. Muskelin is implicated as an isoform-specific anchoring protein for the prostaglandin EP3 receptor, and contains one LisH domain, a CTLH domain, six Kelch repeats and an N-terminal discoidin-like domain through which it self-associates via a head-to-tail mechanism. Human and mouse Muskelin share 98% amino acid seqeuence homology and are encoded by genes located on chromosomes 7q32.3 and 6 A3.3, respectively. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is MKLN1 Polyclonal Antibody (bs-13655R) validated for, and at what dilution?
bs-13655R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-13655R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MKLN1 Polyclonal Antibody (bs-13655R)?
KLH conjugated synthetic peptide derived from human MKLN1 Immunogen range: 301-400/735.