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PAG1 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-PAG1 Polyclonal Antibody, Unconjugated (bs-13695R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-PAG1 Polyclonal Antibody, Unconjugated (bs-13695R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse small intestine; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-PAG1 Polyclonal Antibody, Unconjugated (bs-13695R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse small intestine; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-PAG1 Polyclonal Antibody, Unconjugated (bs-13695R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Mouse bone lysates probed with PAG1 Polyclonal Antibody, Unconjugated (bs-13695R) at 1:300 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37\u02daC.
Mouse bone lysates probed with PAG1 Polyclonal Antibody, Unconjugated (bs-13695R) at 1:300 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37\u02daC.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Spleen tissue lysates; Lane 3: Rat Cerebrum tissue lysates probed with PAG1 Polyclonal Antibody, Unconjugated (bs-13695R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Spleen tissue lysates; Lane 3: Rat Cerebrum tissue lysates probed with PAG1 Polyclonal Antibody, Unconjugated (bs-13695R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • FCM
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Horse
  • Rabbit
Overview
Catalog # bs-13695R
Product Name PAG1 Polyclonal Antibody
Applications
WB ELISA FCM IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Horse, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PAG1
Immunogen Range 331-432/432
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 55824
Swiss Prot Q9NWQ8
Subcellular location Cell membrane
Synonyms CBP; PAG; Phosphoprotein associated with glycosphingolipid-enriched microdomains 1; Csk-binding protein; Transmembrane adapter protein PAG; Transmembrane phosphoprotein Cbp; PAG1
Background Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is PAG1 Polyclonal Antibody (bs-13695R) validated for, and at what dilution?

bs-13695R is validated for WB (1:300-5000); ELISA (1:500-1000); FCM (1:20-100); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PAG1 Polyclonal Antibody (bs-13695R) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Horse, Rabbit.

How should bs-13695R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PAG1 Polyclonal Antibody (bs-13695R)?

KLH conjugated synthetic peptide derived from human PAG1 Immunogen range: 331-432/432.

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