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PL6 Polyclonal Antibody, RBITC Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-13697R-RBITC-100uL
Product Name PL6 Polyclonal Antibody, RBITC Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PL6
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Cell membrane
Synonyms TMEM115;transmembrane protein 115; PP6; Placental protein 6.
Background Transmembrane proteins contain transmembrane domains that are usually characterized by alpha-helical structures. Transmembrane proteins exist as thermodynamically stable hetero- and homodimers that interact with the lipid bilayer and are involved in both material exchange and communication between the cell and the environment. PL6 is a 351 amino acid multi-pass membrane protein that is highly expressed in kidney and skeletal muscle with lower levels of expression detected in liver, placenta, prancreas, lung, heart and brain. PL6 contains one phosphoserine residue and several transmembrane domains, suggesting that it may participate in protein exchange and signaling events between cells.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is PL6 Polyclonal Antibody, RBITC Conjugated (bs-13697R-RBITC) validated for, and at what dilution?

bs-13697R-RBITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-13697R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PL6 Polyclonal Antibody, RBITC Conjugated (bs-13697R-RBITC)?

KLH conjugated synthetic peptide derived from human PL6

What are the excitation and emission wavelengths of the RBITC conjugate (bs-13697R-RBITC)?

RBITC on bs-13697R-RBITC: excitation / emission 540nm/625nm.

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