RAGEF2 Polyclonal Antibody, Biotin Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-13698R-Biotin |
| Product Name | RAGEF2 Polyclonal Antibody, Biotin Conjugated |
| Applications |
ELISA
IHC-P
IHC-F
|
| Predicted Reactivity | Human, Rabbit |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RAGEF2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | DKFZp667N084; DKFZp686I15116; KIA001LB; PDZ domain containing guanine nucleotide exchange factor GEF 2; PDZ domain containing guanine nucleotide exchange factor 2; PDZ domain containing guanine nucleotide exchange factor I; PDZ domain-containing guanine nucleotide exchange factor 2; PDZ GEF 2; PDZ GEF2; PDZ-GEF2; PDZGEF 2; PDZGEF2; RA GEF 2; RA GEF2; RA-GEF-2; RAGEF 2; Rap guanine nucleotide exchange factor GEF 6; Rap guanine nucleotide exchange factor 2; Rap guanine nucleotide exchange factor 6; RAPGEF6; RPGF6_HUMAN. |
| Background | RAPGEF6 is a guanine nucleotide exchange factor (GEF) that is expressed in a variety of tissues. Localizing to the cytoplasm and translocated to the plasma membrane upon ligand binding, RAPGEF6 contains an N-terminal Ras-GEF domain, a cyclic nucleotide monophosphate-binding domain, a PDZ (PSD-95/DlgA/ZO-1) domain, a Ras-associating (RA) domain and a Ras exchanger motif. RAPGEF6 is closely related to RAPGEF2 and both proteins exhibit GEF activity specific towards Rap 1 and Rap 2. In addition, RAPGEF6 is capable of binding to M-Ras via its RA domain. Due to alternative splicing events, two additional isoforms exist for RAPGEF6, namely PDZ-GEF2A and PDZ-GEF2B. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is RAGEF2 Polyclonal Antibody, Biotin Conjugated (bs-13698R-Biotin) validated for, and at what dilution?
bs-13698R-Biotin is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-13698R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RAGEF2 Polyclonal Antibody, Biotin Conjugated (bs-13698R-Biotin)?
KLH conjugated synthetic peptide derived from human RAGEF2