CDH12 Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13715R-HRP |
| Product Name | CDH12 Polyclonal Antibody, HRP Conjugated |
| Applications |
ELISA
IHC-P
IHC-F
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CDH12 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cell membrane |
| Synonyms | Br cadherin; BR-cadherin; Brain cadherin; CAD12_HUMAN; cadherin 12 N cadherin 2; Cadherin 12; Cadherin 12 precursor; Cadherin 12 type 2 N cadherin 2; Cadherin 12 type 2; Cadherin neural type 2; Cadherin-12; CDH12; CDHB; FLJ34857; N cadherin 2; N-cadherin 2; Neural type cadherin 2; Neuronal cadherin 2. |
| Background | The cadherins are a family of Ca++-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morpho-genesis (1-4). Cadherins each contain a large extracellular domain at the amino terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity (5,6). The relatively short carboxy terminal, intracellular domain interacts with a variety of cytoplasmic proteins, including catenin b, to regulate cadherin function (7). BR-cadherin (for brain-cadherin, also designated cadherin-12 or N-cadherin 2) is expressed specifically in neurons in the central nervous system and is thought to be involved in neuronal development (8). |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is CDH12 Polyclonal Antibody, HRP Conjugated (bs-13715R-HRP) validated for, and at what dilution?
bs-13715R-HRP is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CDH12 Polyclonal Antibody, HRP Conjugated (bs-13715R-HRP) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.
How should bs-13715R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CDH12 Polyclonal Antibody, HRP Conjugated (bs-13715R-HRP)?
KLH conjugated synthetic peptide derived from human CDH12