PCDH10 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13723R-RBITC |
| Product Name | PCDH10 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Cow, Sheep, Pig |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PCDH10 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 57575 |
| Swiss Prot | Q9P2E7 |
| Subcellular location | Cell membrane |
| Synonyms | PCDH19; OL-PCDH; Protocadherin-1; PCDH1; KIAA14 |
| Background | Protocadherins are a large family of cadherin-like cell adhesion proteins that are involved in the establishment and maintenance of neuronal connections in the brain. There are three protocadherin gene clusters, designated alpha, beta and gamma, all of which contain multiple tandemly arranged genes. PCDH10 (protocadherin 10), also known as PCDH19 or OL-PCDH, is a 1,040 amino acid single-pass type I membrane protein that contains six cadherin domains and one transmembrane domain. Expressed at moderate levels in brain, ovary and testis and present at lower levels in all other tissues, PCDH10 functions as a calcium-dependent cell-adhesion protein that may function as a tumor suppressor. When underexpressed, PCDH10 is associated with the progression of various carcinomas, including gastric cancer. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PCDH10 Polyclonal Antibody, RBITC Conjugated (bs-13723R-RBITC) validated for, and at what dilution?
bs-13723R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PCDH10 Polyclonal Antibody, RBITC Conjugated (bs-13723R-RBITC) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Pig.
How should bs-13723R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PCDH10 Polyclonal Antibody, RBITC Conjugated (bs-13723R-RBITC)?
KLH conjugated synthetic peptide derived from human PCDH10
What are the excitation and emission wavelengths of the RBITC conjugate (bs-13723R-RBITC)?
RBITC on bs-13723R-RBITC: excitation / emission 540nm/625nm.