SHROOM1 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13735R-Cy3 |
| Product Name | SHROOM1 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SHROOM1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm |
| Synonyms | Apical protein 2; APXL2; Protein Shroom1; SHRM1_HUMAN; Shroom family member 1; Shroom1. |
| Background | SHROOM1 is an 852 amino acid protein that contains one ASD1 domain and one ASD2 domain. Localized to both the cytoplasm and the cytoskeleton, SHROOM1 interacts with Actin and is thought to be involved in microtubule assembly during cell elongation, possibly playing a role in the development of the nervous system. Multiple isoforms of SHROOM1 exist due to alternative splicing events. The gene encoding SHROOM1 maps to human chromosome 5, which contains 181 million base pairs and comprises nearly 6% of the human genome. Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome, while deletion of the q arm or of chromosome 5 altogether is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SHROOM1 Polyclonal Antibody, Cy3 Conjugated (bs-13735R-Cy3) validated for, and at what dilution?
bs-13735R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SHROOM1 Polyclonal Antibody, Cy3 Conjugated (bs-13735R-Cy3) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep.
How should bs-13735R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SHROOM1 Polyclonal Antibody, Cy3 Conjugated (bs-13735R-Cy3)?
KLH conjugated synthetic peptide derived from human SHROOM1
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-13735R-Cy3)?
Cy3 on bs-13735R-Cy3: excitation / emission 512,550nm/570,615nm.