CD99L2 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-13742R-Cy5 |
| Product Name | CD99L2 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CD99L2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 83692 |
| Swiss Prot | Q8TCZ2 |
| Subcellular location | Cell membrane |
| Synonyms | CD99B; MIC2L1; CD99 antigen-like protein 2; MIC2-like protein 1; CD99; CD99L2; UNQ1964/PRO4486 |
| Background | Plays a role in a late step of leukocyte extravasation helping cells to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1 (By similarity). Homophilic adhesion molecule, but these interactions may not be required for cell aggregation (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CD99L2 Polyclonal Antibody, Cy5 Conjugated (bs-13742R-Cy5) validated for, and at what dilution?
bs-13742R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD99L2 Polyclonal Antibody, Cy5 Conjugated (bs-13742R-Cy5) react with?
Tested reactivity: Human.
How should bs-13742R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD99L2 Polyclonal Antibody, Cy5 Conjugated (bs-13742R-Cy5)?
KLH conjugated synthetic peptide derived from human CD99L2
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-13742R-Cy5)?
Cy5 on bs-13742R-Cy5: excitation / emission 625,650nm/670nm.