Claudin 23 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13746R-Cy7 |
| Product Name | Claudin 23 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Rabbit |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Claudin 23 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cell membrane |
| Synonyms | 2310014B08Rik; Claudin23; CLDN 23; CLDN23; CLDNL. |
| Background | The claudin superfamily consists of structurally related proteins that are important structural and functional components of tight junctions. Claudin-23, also known as CLDN23 or CLDNL, is a 292 amino acid multi-pass membrane protein that localizes to cell junctions and belongs to the claudin family. Expressed in stomach and placenta, as well as in germinal center B-cells, claudin-23 is thought to exhibit calcium-dependent cell-adhesion activity through which it plays an essential role in tight junction-specific obliteration of the intercellular space. Human claudin-23 shares 80% sequence similarity with its mouse counterpart, suggesting a conserved role between species. Overexpression of claudin-23 is associated with colon tumors, implicating claudin-23 as a possible metastasis factor. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Claudin 23 Polyclonal Antibody, Cy7 Conjugated (bs-13746R-Cy7) validated for, and at what dilution?
bs-13746R-Cy7 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Claudin 23 Polyclonal Antibody, Cy7 Conjugated (bs-13746R-Cy7) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Rabbit.
How should bs-13746R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Claudin 23 Polyclonal Antibody, Cy7 Conjugated (bs-13746R-Cy7)?
KLH conjugated synthetic peptide derived from human Claudin 23
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-13746R-Cy7)?
Cy7 on bs-13746R-Cy7: excitation / emission 743nm/767nm.