Claudin 15 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-13753R-RBITC |
| Product Name | Claudin 15 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Claudin 15 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cell membrane |
| Synonyms | Claudin-15; CLD15_HUMAN; CLDN15. |
| Background | The claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the claudins, Occludin and Junction adhesion molecules. Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is often highly restricted to specfic regions of different tissues and may have an important role in transcellular transport through tight junctions. Claudin-15 is a 228 amino acid multi-pass membrane protein that belongs to the claudin family and plays an important role in cell-adhesion activity and tight junction-specific events. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Claudin 15 Polyclonal Antibody, RBITC Conjugated (bs-13753R-RBITC) validated for, and at what dilution?
bs-13753R-RBITC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Claudin 15 Polyclonal Antibody, RBITC Conjugated (bs-13753R-RBITC) react with?
Tested reactivity: Human.
How should bs-13753R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Claudin 15 Polyclonal Antibody, RBITC Conjugated (bs-13753R-RBITC)?
KLH conjugated synthetic peptide derived from human Claudin 15
What are the excitation and emission wavelengths of the RBITC conjugate (bs-13753R-RBITC)?
RBITC on bs-13753R-RBITC: excitation / emission 540nm/625nm.