Help

NUDCD3 Polyclonal Antibody, APC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Pig
Overview
Catalog # bs-13766R-APC
Product Name NUDCD3 Polyclonal Antibody, APC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Rat
Predicted Reactivity Mouse, Pig
Specifications
Conjugation APC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NUDCD3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 23386
Swiss Prot Q8IVD9
Subcellular location Nucleus
Synonyms NudC domain-containing protein 3; NUDCD3; KIAA1068
Background NUDCD3 functions to maintain the stability of dynein intermediate chain. Depletion of NUDCD3 results in aggregation and degradation of dynein intermediate chain, mislocalization of the dynein complex from kinetochores, spindle microtubules, and spindle poles, and loss of gamma-tubulin from spindle poles. NUDCD3 levels increase after the G1/S transition.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is NUDCD3 Polyclonal Antibody, APC Conjugated (bs-13766R-APC) validated for, and at what dilution?

bs-13766R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NUDCD3 Polyclonal Antibody, APC Conjugated (bs-13766R-APC) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Pig.

How should bs-13766R-APC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NUDCD3 Polyclonal Antibody, APC Conjugated (bs-13766R-APC)?

KLH conjugated synthetic peptide derived from human NUDCD3

What are the excitation and emission wavelengths of the APC conjugate (bs-13766R-APC)?

APC on bs-13766R-APC: excitation / emission 650nm/660nm.