NUDCD3 Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13766R-PerCP-Cy5.5 |
| Product Name | NUDCD3 Polyclonal Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Pig |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NUDCD3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23386 |
| Swiss Prot | Q8IVD9 |
| Subcellular location | Nucleus |
| Synonyms | NudC domain-containing protein 3; NUDCD3; KIAA1068 |
| Background | NUDCD3 functions to maintain the stability of dynein intermediate chain. Depletion of NUDCD3 results in aggregation and degradation of dynein intermediate chain, mislocalization of the dynein complex from kinetochores, spindle microtubules, and spindle poles, and loss of gamma-tubulin from spindle poles. NUDCD3 levels increase after the G1/S transition. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NUDCD3 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-13766R-PERCP-CY5-5) validated for, and at what dilution?
BS-13766R-PERCP-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NUDCD3 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-13766R-PERCP-CY5-5) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Pig.
How should BS-13766R-PERCP-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NUDCD3 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-13766R-PERCP-CY5-5)?
KLH conjugated synthetic peptide derived from human NUDCD3
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-13766R-PERCP-CY5-5)?
PerCP-Cy5.5 on BS-13766R-PERCP-CY5-5: excitation / emission 482nm/694nm.