phospho-14-3-3 beta + zeta (Ser186 / Ser184) Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13773R-BF594-100uL |
| Product Name | phospho-14-3-3 beta + zeta (Ser186 / Ser184) Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthesised phosphopeptide derived from human 14-3-3 beta + zeta around the phosphorylation site of Ser186 / Ser184 |
| Modification Site | Ser186 / Ser184 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7529 |
| Swiss Prot | P31946 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | 14-3-3 beta + zeta (phospho S184 + S186); 14-3-3 beta + zeta (phospho S186); 14-3-3 beta + zeta (phospho Ser186); p-14-3-3 beta + zeta (Ser186) 14 3 3 protein beta; 14 3 3 protein beta/alpha; 14 3 3 protein zeta/delta; 14 3 3 zeta; HS1; KCIP1; YWHAB; YWHAZ. |
| Background | Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation amoung many other functions. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-14-3-3 beta + zeta (Ser186 / Ser184) Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-13773R-BF594) validated for, and at what dilution?
bs-13773R-BF594 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-14-3-3 beta + zeta (Ser186 / Ser184) Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-13773R-BF594) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, Chicken.
How should bs-13773R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-14-3-3 beta + zeta (Ser186 / Ser184) Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-13773R-BF594)?
KLH conjugated synthesised phosphopeptide derived from human 14-3-3 beta + zeta around the phosphorylation site of Ser186 / Ser184
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-13773R-BF594)?
AbBy Fluor-594 on bs-13773R-BF594: excitation / emission 590nm/617nm.