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CELF3 Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Cow
  • Pig
  • Chicken
Overview
Catalog # bs-13830R-PE
Product Name CELF3 Polyclonal Antibody, PE Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Cow, Pig, Chicken
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CELF3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 11189
Swiss Prot Q5SZQ8
Subcellular location Cytoplasm, Nucleus
Synonyms Bruno-like protein 1; BRUNOL1; CAG repeat protein 4; CAGH4; CELF-3; CELF3; CELF3_HUMAN; CUG-BP- and ETR-3-like factor 3; CUGBP Elav-like family member 3; ELAV-type RNA-binding protein 1; ERDA4; ETR-1; Expanded repeat domain protein CAG/CTG 4; RNA-binding protein BRUNOL-1; TNRC4; Trinucleotide repeat-containing gene 4 protein.
Background RNA-binding protein involved in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA.
Application Dilution
WB 1:300-5000
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is CELF3 Polyclonal Antibody, PE Conjugated (bs-13830R-PE) validated for, and at what dilution?

bs-13830R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CELF3 Polyclonal Antibody, PE Conjugated (bs-13830R-PE) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Pig, Chicken.

How should bs-13830R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CELF3 Polyclonal Antibody, PE Conjugated (bs-13830R-PE)?

KLH conjugated synthetic peptide derived from human CELF3

What are the excitation and emission wavelengths of the PE conjugate (bs-13830R-PE)?

PE on bs-13830R-PE: excitation / emission 496,564nm/578nm.