Chimaerin 2 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13903R-Cy7-100uL |
| Product Name | Chimaerin 2 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Cow, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Chimaerin 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1124 |
| Swiss Prot | P52757 |
| Subcellular location | Cell membrane |
| Synonyms | ARHGAP3; BCH; Beta chimerin; Chimaerin2; Chimerin (chimaerin) 2; Chimerin 2; CHN 2; CHN2; CHIO_HUMAN; MGC138360; Rho GTPase activating protein 3; RHOGAP3. |
| Background | The Chimaerin gene is a member of the chimerin family and encodes a protein with a phorbol-ester/DAG-type zinc finger, an SH2 domain and a Rho-GAP domain. This protein has GTPase-activating protein activity which is regulated by phospholipid binding and binding of diacylglycerol (DAG) induces translocation of the protein from the cytosol to the Golgi apparatus membrane. The protein plays a role in the proliferation and migration of smooth muscle cells. Increased expression of this gene is associated with lymphomas and decreased expression of this gene is associated with high-grade gliomas and breast tumors. Mutations in this gene have been associated with schizophrenia in men. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| Application Dilution | |
| WB | =1:500-2000 |
Frequently asked questions
Which applications is Chimaerin 2 Polyclonal Antibody, Cy7 Conjugated (bs-13903R-Cy7) validated for, and at what dilution?
bs-13903R-Cy7 is validated for WB (WB=1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Chimaerin 2 Polyclonal Antibody, Cy7 Conjugated (bs-13903R-Cy7) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Sheep, Pig, Horse, Chicken.
How should bs-13903R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Chimaerin 2 Polyclonal Antibody, Cy7 Conjugated (bs-13903R-Cy7)?
KLH conjugated synthetic peptide derived from human Chimaerin 2
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-13903R-Cy7)?
Cy7 on bs-13903R-Cy7: excitation / emission 743nm/767nm.