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CHIT1 Polyclonal Antibody, AbBy Fluor-594 Conjugated

Applications

  • WB

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Cow
  • Pig
  • Rabbit
Overview
Catalog # bs-13904R-BF594-100uL
Product Name CHIT1 Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
WB
Reactivity Rat
Predicted Reactivity Human, Mouse, Cow, Pig, Rabbit
Specifications
Conjugation AbBy Fluor-594
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CHIT1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1118
Swiss Prot Q13231
Subcellular location Cytoplasm
Synonyms CHI3; CHIT; CHIT1; CHIT1_HUMAN; CHITD; Chitinase 1; Chitinase 1 (chitotriosidase); Chitinase-1; Chitotriosidase; Chitotriosidase-1; EC 3.2.1.14; FLJ00314; MGC125322; Plasma methylumbelliferyl tetra N acetylchitotetraoside hydrolase.
Background Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2012]
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is CHIT1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-13904R-BF594) validated for, and at what dilution?

bs-13904R-BF594 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CHIT1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-13904R-BF594) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Pig, Rabbit.

How should bs-13904R-BF594 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CHIT1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-13904R-BF594)?

KLH conjugated synthetic peptide derived from human CHIT1

What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-13904R-BF594)?

AbBy Fluor-594 on bs-13904R-BF594: excitation / emission 590nm/617nm.