CHIT1 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13904R-RBITC |
| Product Name | CHIT1 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Cow, Pig, Rabbit |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CHIT1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1118 |
| Swiss Prot | Q13231 |
| Subcellular location | Cytoplasm |
| Synonyms | CHI3; CHIT; CHIT1; CHIT1_HUMAN; CHITD; Chitinase 1; Chitinase 1 (chitotriosidase); Chitinase-1; Chitotriosidase; Chitotriosidase-1; EC 3.2.1.14; FLJ00314; MGC125322; Plasma methylumbelliferyl tetra N acetylchitotetraoside hydrolase. |
| Background | Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2012] |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is CHIT1 Polyclonal Antibody, RBITC Conjugated (bs-13904R-RBITC) validated for, and at what dilution?
bs-13904R-RBITC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CHIT1 Polyclonal Antibody, RBITC Conjugated (bs-13904R-RBITC) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Pig, Rabbit.
How should bs-13904R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CHIT1 Polyclonal Antibody, RBITC Conjugated (bs-13904R-RBITC)?
KLH conjugated synthetic peptide derived from human CHIT1
What are the excitation and emission wavelengths of the RBITC conjugate (bs-13904R-RBITC)?
RBITC on bs-13904R-RBITC: excitation / emission 540nm/625nm.