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CHST10 Polyclonal Antibody, AbBy Fluor-555 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-13929R-BF555
Product Name CHST10 Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation AbBy Fluor-555
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CHST10
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9186
Swiss Prot O43529
Subcellular location Cytoplasm
Synonyms Carbohydrate sulfotransferase 10; CHST10; HNK-1 sulfotransferase; HNK-1ST; HNK1ST; HuHNK-1ST; CHST10
Background Catalyzes the transfer of sulfate to position 3 of terminal glucuronic acid of both protein- and lipid-linked oligosaccharides. Participates in biosynthesis of HNK-1 carbohydrate structure, a sulfated glucuronyl-lactosaminyl residue carried by many neural recognition molecules, which is involved in cell interactions during ontogenetic development and in synaptic plasticity in the adult. May be indirectly involved in synapse plasticity of the hippocampus, via its role in HNK-1 biosynthesis.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CHST10 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-13929R-BF555) validated for, and at what dilution?

bs-13929R-BF555 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CHST10 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-13929R-BF555) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken.

How should bs-13929R-BF555 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CHST10 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-13929R-BF555)?

KLH conjugated synthetic peptide derived from human CHST10

What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-13929R-BF555)?

AbBy Fluor-555 on bs-13929R-BF555: excitation / emission 553nm/568nm.