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CHST11 Polyclonal Antibody, Cy5.5 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Chicken
Overview
Catalog # bs-13930R-Cy5.5
Product Name CHST11 Polyclonal Antibody, Cy5.5 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Sheep, Pig, Chicken
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CHST11
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 50515
Swiss Prot Q9NPF2
Subcellular location Cytoplasm
Synonyms C4S-1; C4ST 1; C4ST; C4ST-1; C4ST1; Carbohydrate (chondroitin 4) sulfotransferase 11; Carbohydrate sulfotransferase 11; Chondroitin 4 O sulfotransferase 1; Chondroitin 4 sulfotransferase; Chondroitin 4-O-sulfotransferase 1; Chondroitin 4-sulfotransferase 1; CHST 11; Chst11; CHSTB_HUMAN; HSA269537; IgH/CHST11 fusion.
Background Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues in desulfated dermatan sulfate. Preferentially sulfates in GlcA->GalNAc unit than in IdoA->GalNAc unit. Does not form 4, 6-di-O-sulfated GalNAc when chondroitin sulfate C is used as an acceptor.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CHST11 Polyclonal Antibody, Cy5.5 Conjugated (BS-13930R-CY5-5) validated for, and at what dilution?

BS-13930R-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CHST11 Polyclonal Antibody, Cy5.5 Conjugated (BS-13930R-CY5-5) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Chicken.

How should BS-13930R-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CHST11 Polyclonal Antibody, Cy5.5 Conjugated (BS-13930R-CY5-5)?

KLH conjugated synthetic peptide derived from human CHST11

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-13930R-CY5-5)?

Cy5.5 on BS-13930R-CY5-5: excitation / emission 675nm/694nm.