CHST3 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-13935R-BF488 |
| Product Name | CHST3 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CHST3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9469 |
| Swiss Prot | Q7LGC8 |
| Subcellular location | Cytoplasm |
| Synonyms | Carbohydrate sulfotransferase 3; CHST3; Chondroitin 6-O-sulfotransferase 1; C6ST-1; Chondroitin 6-sulfotransferase; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0; GST-0 |
| Background | Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues of keratan sulfate, another glycosaminoglycan, and the Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. May play a role in the maintenance of naive T-lymphocytes in the spleen. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CHST3 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-13935R-BF488) validated for, and at what dilution?
bs-13935R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CHST3 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-13935R-BF488) react with?
Tested reactivity: Human, Mouse.
How should bs-13935R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CHST3 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-13935R-BF488)?
KLH conjugated synthetic peptide derived from human CHST3
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-13935R-BF488)?
AbBy Fluor-488 on bs-13935R-BF488: excitation / emission 499nm/519nm.