COP1 (Ser387) Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-13976r-100uL |
| Product Name | COP1 (Ser387) Polyclonal Antibody |
| Applications |
IHC-P
IHC-F
IF(ICC)
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthesised phosphopeptide derived from human COP1 around the phosphorylation site of Ser387 |
| Modification Site | Ser387 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 64326 |
| Swiss Prot | Q8NHY2 |
| Synonyms | COP1 (phospho S387); p-COP1 (phospho S387); Constitutive photomorphogenesis protein 1 homolog; COP; E3 ubiquitin-protein ligase RFWD2; hCOP1; RFWD2; RFWD2_HUMAN; RNF200; ring finger and WD repeat domain 2; RING finger and WD repeat domain protein 2; RING finger protein 200. |
| Background | COP1 is an E3 ubiquitin ligase protein that mediates ubiquitination and degradation of target proteins such as c-Jun and p53. It is a component of the DCX DET1-COP1 ubiquitin ligase complex which consists of RBX1, DET1, DDB1, CUL4A and COP1. Localizing to the cytoplasm and to the nucleus, COP1 is primarily expressed in testis, placenta, heart and skeletal muscle. COP1 is a potent inhibitor of p53-dependent transcription and apoptosis but, when phosphorylated by Atm (ataxia telangiectasia mutated) in response to DNA damage, the COP1-p53 complex is disrupted and p53 is allowed to exert its pro-apoptotic properties. In ovarian and breast cancers, COP1 is overexpressed, suggesting a role for COP1 in tumorigenesis. |
| Application Dilution | |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(ICC) | 1:50-200 |
| IF | |
Frequently asked questions
Which applications is COP1 (Ser387) Polyclonal Antibody (bs-13976R) validated for, and at what dilution?
bs-13976R is validated for IHC-P (1:200-400); IHC-F (1:100-500); IF(ICC) (1:50-200); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does COP1 (Ser387) Polyclonal Antibody (bs-13976R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-13976R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise COP1 (Ser387) Polyclonal Antibody (bs-13976R)?
KLH conjugated synthesised phosphopeptide derived from human COP1 around the phosphorylation site of Ser387