COP1 (Ser387) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-13976r-cy3 |
| Product Name | COP1 (Ser387) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(ICC)
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthesised phosphopeptide derived from human COP1 around the phosphorylation site of Ser387 |
| Modification Site | Ser387 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 64326 |
| Swiss Prot | Q8NHY2 |
| Synonyms | COP1 (phospho S387); p-COP1 (phospho S387); Constitutive photomorphogenesis protein 1 homolog; COP; E3 ubiquitin-protein ligase RFWD2; hCOP1; RFWD2; RFWD2_HUMAN; RNF200; ring finger and WD repeat domain 2; RING finger and WD repeat domain protein 2; RING finger protein 200. |
| Background | COP1 is an E3 ubiquitin ligase protein that mediates ubiquitination and degradation of target proteins such as c-Jun and p53. It is a component of the DCX DET1-COP1 ubiquitin ligase complex which consists of RBX1, DET1, DDB1, CUL4A and COP1. Localizing to the cytoplasm and to the nucleus, COP1 is primarily expressed in testis, placenta, heart and skeletal muscle. COP1 is a potent inhibitor of p53-dependent transcription and apoptosis but, when phosphorylated by Atm (ataxia telangiectasia mutated) in response to DNA damage, the COP1-p53 complex is disrupted and p53 is allowed to exert its pro-apoptotic properties. In ovarian and breast cancers, COP1 is overexpressed, suggesting a role for COP1 in tumorigenesis. |
| Application Dilution | |
| IF(ICC) | 1:50-200 |
| IF | |
Frequently asked questions
Which applications is COP1 (Ser387) Polyclonal Antibody, Cy3 Conjugated (bs-13976R-Cy3) validated for, and at what dilution?
bs-13976R-Cy3 is validated for IF(ICC) (1:50-200); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does COP1 (Ser387) Polyclonal Antibody, Cy3 Conjugated (bs-13976R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-13976R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise COP1 (Ser387) Polyclonal Antibody, Cy3 Conjugated (bs-13976R-Cy3)?
KLH conjugated synthesised phosphopeptide derived from human COP1 around the phosphorylation site of Ser387
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-13976R-Cy3)?
Cy3 on bs-13976R-Cy3: excitation / emission 512,550nm/570,615nm.