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CORO7 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CORO7 Polyclonal Antibody, Unconjugated (bs-13999R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CORO7 Polyclonal Antibody, Unconjugated (bs-13999R) at 1:400 overnight at 4\u00b0C, DAB staining.
MDA-MB-231 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (CORO7) polyclonal Antibody, Unconjugated (bs-13999R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
MDA-MB-231 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (CORO7) polyclonal Antibody, Unconjugated (bs-13999R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Bioss unconjugated antibody vial

Applications

  • IHC-P
  • IHC-F
  • IF
  • ICC/IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-13999R
Product Name CORO7 Polyclonal Antibody
Applications
IHC-P IHC-F IF ICC/IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CORO7
Immunogen Range 160-210/925
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 79585
Swiss Prot P57737
Synonyms Clipin-E; CORO6; CORO7_HUMAN; Coronin, actin binding protein 6; Coronin-6; Coronin-like protein E; FLJ14871; PP1009; PP1782; PP1881.
Background Coronins, such as CORO7, constitute an evolutionarily conserved family of WD-repeat actin-binding proteins. CORO7 plays a role in Golgi complex morphology and function (Rybakin et al., 2004, 2006 [PubMed 15327992] [PubMed 16905771]).[supplied by OMIM, Mar 2008]
Application Dilution
IHC-P
IHC-F IFIHC-P1:50-200
IF
ICC/IF

Frequently asked questions

Which applications is CORO7 Polyclonal Antibody (bs-13999R) validated for, and at what dilution?

bs-13999R is validated for IHC-P; IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CORO7 Polyclonal Antibody (bs-13999R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-13999R be stored?

Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CORO7 Polyclonal Antibody (bs-13999R)?

KLH conjugated synthetic peptide derived from human CORO7 Immunogen range: 160-210/925.

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