E2F4 Polyclonal Antibody

Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-E2F4 Polyclonal Antibody, Unconjugated (bs-1399R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Lane 1: Human A431 cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human HL-60 cell lysates probed with E2F4 Polyclonal Antibody, Unconjugated (bs-1399R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.$

Lane 1: Human A431 cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human HL-60 cell lysates probed with E2F4 Polyclonal Antibody, Unconjugated (bs-1399R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-1399r-100ul
Product Name	E2F4 Polyclonal Antibody
Applications	WB, IHC-P, IF(IHC-P)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human E2F4
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	1874
Synonyms	E2F 4; E2F transcription factor 4; E2F transcription factor 4 p107/p130 binding; p107/p130 binding protein; Transcription factor E2F 4; Transcription factor E2F4; E2F4_HUMAN.
Background	E2Fs are DNA binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F4 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200