SREBP1 Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1402R-PE-Cy5-100uL |
| Product Name | SREBP1 Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Sheep, Chicken |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SREBP-1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6720, 6721 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Sterol regulatory element binding protein 1; ADD 1; BHLHD1; D630008H06; SREBF 1; SREBF1; SREBP 1; SREBP1; SREBP 1c; Sterol regulatory element binding protein 1; Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1; Sterol regulatory element binding transcription factor 1. |
| Background | Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). Isoform SREBP-1A is much more active than isoform SREBP-1C in stimulating transcription from SRE-1-containing promoters. [SUBUNIT] Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. [SUBCELLULAR LOCATION] Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane; Multi-pass membrane protein. Note=Moves from the endoplasmic reticulum to the Golgi in the absence of sterols. [SUBCELLULAR LOCATION] Processed sterol regulatory element-binding protein 1: Nucleus. Belongs to the SREBP family. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is SREBP1 Polyclonal Antibody, PE-Cy5 Conjugated (bs-1402R-PE-Cy5) validated for, and at what dilution?
bs-1402R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SREBP1 Polyclonal Antibody, PE-Cy5 Conjugated (bs-1402R-PE-Cy5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Sheep, Chicken.
How should bs-1402R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SREBP1 Polyclonal Antibody, PE-Cy5 Conjugated (bs-1402R-PE-Cy5)?
KLH conjugated synthetic peptide derived from human SREBP-1
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-1402R-PE-Cy5)?
PE-Cy5 on bs-1402R-PE-Cy5: excitation / emission 488nm/670nm.