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CRB1 Polyclonal Antibody, AbBy Fluor-405 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bs-14045R-BF405
Product Name CRB1 Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Specifications
Conjugation AbBy Fluor-405
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CRB1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 23418
Swiss Prot P82279
Subcellular location Cytoplasm, Cell membrane
Synonyms CRB1; CRUM1_HUMAN; Protein crumbs homolog 1.
Background This gene encodes a protein which is similar to the Drosophila crumbs protein and localizes to the inner segment of mammalian photoreceptors. In Drosophila crumbs localizes to the stalk of the fly photoreceptor and may be a component of the molecular scaffold that controls proper development of polarity in the eye. Mutations in this gene are associated with a severe form of retinitis pigmentosa, RP12, and with Leber congenital amaurosis. Alternate splicing results in multiple transcript variants, some protein coding and some non-protein coding.[provided by RefSeq, Apr 2012]
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CRB1 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-14045R-BF405) validated for, and at what dilution?

bs-14045R-BF405 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CRB1 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-14045R-BF405) react with?

Tested reactivity: Human.

How should bs-14045R-BF405 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CRB1 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-14045R-BF405)?

KLH conjugated synthetic peptide derived from human CRB1

What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-14045R-BF405)?

AbBy Fluor-405 on bs-14045R-BF405: excitation / emission 401nm/421nm.