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Creatine kinase MT Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Sheep
  • Pig
  • Chicken
Overview
Catalog # bs-14050R-FITC
Product Name Creatine kinase MT Polyclonal Antibody, FITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Sheep, Pig, Chicken
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Creatine kinase MT
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1159
Swiss Prot P12532
Subcellular location Cell membrane
Synonyms Acidic type mitochondrial creatine kinase; CKMT; CKMT1; CKMT1A; CKMT1B; Creatine kinase mitochondrial 1 ubiquitous; Creatine kinase ubiquitous mitochondrial; Mia CK; Ubiquitous mitochondrial creatine kinase; UMTCK; KCRU_HUMAN; CKMT2; Basic-type mitochondrial creatine kinase; CKB; Creatine kinase B-type; Brain creatine kinase; CKM; Creatine kinase M-type.
Background Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Creatine kinase MT Polyclonal Antibody, FITC Conjugated (bs-14050R-FITC) validated for, and at what dilution?

bs-14050R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Creatine kinase MT Polyclonal Antibody, FITC Conjugated (bs-14050R-FITC) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Sheep, Pig, Chicken.

How should bs-14050R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Creatine kinase MT Polyclonal Antibody, FITC Conjugated (bs-14050R-FITC)?

KLH conjugated synthetic peptide derived from human Creatine kinase MT

What are the excitation and emission wavelengths of the FITC conjugate (bs-14050R-FITC)?

FITC on bs-14050R-FITC: excitation / emission 494nm/518nm.