Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch
Help

HNF1A Polyclonal Antibody

Formalin-fixed and paraffin embedded human kidney labeled with Anti-HNF1\/TCF1\/HNF1A Polyclonal Antibody, Unconjugated (bs-1405R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human kidney labeled with Anti-HNF1\/TCF1\/HNF1A Polyclonal Antibody, Unconjugated (bs-1405R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (HNF1A) polyclonal Antibody, Unconjugated (bs-1405R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (HNF1A) polyclonal Antibody, Unconjugated (bs-1405R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF
  • Flow-Cyt
  • ICC/IF

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Chicken
  • Zebrafish
Overview
Catalog # bs-1405R
Product Name HNF1A Polyclonal Antibody
Applications
WB IHC-P IHC-F IF FLOW-CYT ICC/IF
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Sheep, Pig, Chicken, Zebrafish
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from rat HNF1
Immunogen Range 201-350/628
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 24817
Swiss Prot P15257
Subcellular location Nucleus
Synonyms HNF1; Lfb1; Tcf1; LF-B1; Hepatocyte nuclear factor 1-alpha; HNF-1-alpha; HNF-1A; Liver-specific transcription factor LF-B1; Transcription factor 1; TCF-1; Hnf1a; Hnf-1
Background Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
Application Dilution
WB 1:300-5000
IHC-P ELISA1:500-1000
IHC-F FCM1:20-100
IF IHC-P1:200-400
Flow-Cyt
ICC/IF

Frequently asked questions

Which applications is HNF1A Polyclonal Antibody (bs-1405R) validated for, and at what dilution?

bs-1405R is validated for WB (1:300-5000); ELISA (1:500-1000); FCM (1:20-100); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HNF1A Polyclonal Antibody (bs-1405R) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Chicken, Zebrafish.

How should bs-1405R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HNF1A Polyclonal Antibody (bs-1405R)?

KLH conjugated synthetic peptide derived from rat HNF1 Immunogen range: 201-350/628.

Search our catalog of hundreds of thousands of products