HNF1A Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1405R-Cy5 |
| Product Name | HNF1A Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig, Chicken, Zebrafish |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from rat HNF1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 24817 |
| Swiss Prot | P15257 |
| Subcellular location | Nucleus |
| Synonyms | HNF1; Lfb1; Tcf1; LF-B1; Hepatocyte nuclear factor 1-alpha; HNF-1-alpha; HNF-1A; Liver-specific transcription factor LF-B1; Transcription factor 1; TCF-1; Hnf1a; Hnf-1 |
| Background | Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | FCM1:20-100 |
Frequently asked questions
Which applications is HNF1A Polyclonal Antibody, Cy5 Conjugated (bs-1405R-Cy5) validated for, and at what dilution?
bs-1405R-Cy5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HNF1A Polyclonal Antibody, Cy5 Conjugated (bs-1405R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Chicken, Zebrafish.
How should bs-1405R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HNF1A Polyclonal Antibody, Cy5 Conjugated (bs-1405R-Cy5)?
KLH conjugated synthetic peptide derived from rat HNF1
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-1405R-Cy5)?
Cy5 on bs-1405R-Cy5: excitation / emission 625,650nm/670nm.