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HNF1A Polyclonal Antibody, HRP Conjugated

Applications

  • WB
  • IHC-P
  • IHC-F

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Chicken
  • Zebrafish
Overview
Catalog # bs-1405R-HRP
Product Name HNF1A Polyclonal Antibody, HRP Conjugated
Applications
WB IHC-P IHC-F
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Sheep, Pig, Chicken, Zebrafish
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from rat HNF1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 24817
Swiss Prot P15257
Subcellular location Nucleus
Synonyms HNF1; Lfb1; Tcf1; LF-B1; Hepatocyte nuclear factor 1-alpha; HNF-1-alpha; HNF-1A; Liver-specific transcription factor LF-B1; Transcription factor 1; TCF-1; Hnf1a; Hnf-1
Background Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
Application Dilution
WB 1:300-5000
IHC-P ELISA1:500-1000
IHC-F IHC-P1:200-400

Frequently asked questions

Which applications is HNF1A Polyclonal Antibody, HRP Conjugated (bs-1405R-HRP) validated for, and at what dilution?

bs-1405R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HNF1A Polyclonal Antibody, HRP Conjugated (bs-1405R-HRP) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Chicken, Zebrafish.

How should bs-1405R-HRP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HNF1A Polyclonal Antibody, HRP Conjugated (bs-1405R-HRP)?

KLH conjugated synthetic peptide derived from rat HNF1