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HNF1A Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Chicken
  • Zebrafish
Overview
Catalog # bs-1405R-PE-100uL
Product Name HNF1A Polyclonal Antibody, PE Conjugated
Applications
WB IF
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Sheep, Pig, Chicken, Zebrafish
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from rat HNF1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 24817
Swiss Prot P15257
Subcellular location Nucleus
Synonyms HNF1; Lfb1; Tcf1; LF-B1; Hepatocyte nuclear factor 1-alpha; HNF-1-alpha; HNF-1A; Liver-specific transcription factor LF-B1; Transcription factor 1; TCF-1; Hnf1a; Hnf-1
Background Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
Application Dilution
WB 1:300-5000
IF FCM1:20-100

Frequently asked questions

Which applications is HNF1A Polyclonal Antibody, PE Conjugated (bs-1405R-PE) validated for, and at what dilution?

bs-1405R-PE is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HNF1A Polyclonal Antibody, PE Conjugated (bs-1405R-PE) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Chicken, Zebrafish.

How should bs-1405R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HNF1A Polyclonal Antibody, PE Conjugated (bs-1405R-PE)?

KLH conjugated synthetic peptide derived from rat HNF1

What are the excitation and emission wavelengths of the PE conjugate (bs-1405R-PE)?

PE on bs-1405R-PE: excitation / emission 496,564nm/578nm.