CRISP10 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-14060R-BF680 |
| Product Name | CRISP10 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CRISP10 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 83690 |
| Swiss Prot | Q9H336 |
| Subcellular location | Cytoplasm |
| Synonyms | CRISPLD1; CocoaCrisp; CRISP 10; CRISP-10; CRISP10; CRISPLD 1; Crispld1; CRLD1_HUMAN; Cysteine rich secretory protein 10; Cysteine rich secretory protein LCCL domain containing 1; Cysteine-rich secretory protein 10; Cysteine-rich secretory protein LCCL domain-containing 1; LCCL domain containing cysteine rich secretory protein 1; LCCL domain-containing cysteine-rich secretory protein 1; LCRISP1; Trypsin inhibitor Hl; UNQ342/PRO541. |
| Background | Cysteine-rich secretory proteins (CRISPs) represent a family of evolutionarily conserved proteins which may play a role in the innate immune system and are transcriptionally regulated by androgens in several tissues. CRISP proteins are highly expressed in the mammalian reproductive tract and in the venom secretory ducts of some reptiles. CRISP-10 (cysteine-rich secretory protein 10), also known as CocoaCrisp and Trypsin inhibitor Hl, is a 500 amino acid protein containing 2 LCCL domains, which are thought to function as autonomous folding domains used to construct modular proteins through exon shuffling. CRISP-10 differs from other CRISP proteins in that it does not contain the 10 conserved cysteine residues or ICR domains that are usually conserved throughout the CRISP family. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CRISP10 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-14060R-BF680) validated for, and at what dilution?
bs-14060R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CRISP10 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-14060R-BF680) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Horse, Rabbit.
How should bs-14060R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CRISP10 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-14060R-BF680)?
KLH conjugated synthetic peptide derived from human CRISP10
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-14060R-BF680)?
AbBy Fluor-680 on bs-14060R-BF680: excitation / emission 679nm/702nm.