Crry Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-14070R-BF488 |
| Product Name | Crry Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse Crry |
| Immunogen Range | 51-150/559 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 12946 |
| Swiss Prot | Q64735 |
| Subcellular location | Cell membrane |
| Synonyms | Mcp; Crry; mCRY; Complement component receptor 1-like protein; Complement regulatory protein Crry; Protein p65; Cr1l; Cry |
| Background | Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. Also acts as a decay-accelerating factor, preventing the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Plays a crucial role in early embryonic development by maintaining fetomaternal tolerance. Also acts as a costimulatory factor for T-cells which favors IL-4 secretion. |
| Application Dilution | |
| WB | 1:50-200 |
| IF(IHC-P) | 1:100-500 |
| IF(IHC-F) | 1:200-400 |
| IF(ICC) | 1:500-1000 |
Frequently asked questions
Which applications is Crry Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-14070R-BF488) validated for, and at what dilution?
bs-14070R-BF488 is validated for WB (1:50-200); IF(IHC-P) (1:100-500); IF(IHC-F) (1:200-400); IF(ICC) (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Crry Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-14070R-BF488) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should bs-14070R-BF488 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Crry Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-14070R-BF488)?
KLH conjugated synthetic peptide derived from mouse Crry Immunogen range: 51-150/559.
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-14070R-BF488)?
AbBy Fluor-488 on bs-14070R-BF488: excitation / emission 499nm/519nm.