CTDSP2 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-14099R-Cy7 |
| Product Name | CTDSP2 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CTDSP2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10106 |
| Swiss Prot | O14595 |
| Subcellular location | Nucleus |
| Synonyms | OS4; PSR2; SCP2; Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2; Nuclear LIM interactor-interacting factor 2; NLI-interacting factor 2; Protein OS-4; Small C-terminal domain phosphatase 2; Small CTD phosphatase 2; CTDSP2; NIF2 |
| Background | Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells. May contribute to the development of sarcomas. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CTDSP2 Polyclonal Antibody, Cy7 Conjugated (bs-14099R-Cy7) validated for, and at what dilution?
bs-14099R-Cy7 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CTDSP2 Polyclonal Antibody, Cy7 Conjugated (bs-14099R-Cy7) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-14099R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CTDSP2 Polyclonal Antibody, Cy7 Conjugated (bs-14099R-Cy7)?
KLH conjugated synthetic peptide derived from human CTDSP2
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-14099R-Cy7)?
Cy7 on bs-14099R-Cy7: excitation / emission 743nm/767nm.