CTU2 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-14109R-BF488 |
| Product Name | CTU2 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Cow, Sheep, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CTU2/C16orf84 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 348180 |
| Swiss Prot | Q2VPK5 |
| Subcellular location | Cytoplasm |
| Synonyms | C16orf84; ctu2; CTU2_HUMAN; Cytoplasmic tRNA 2-thiolation protein 2; cytosolic thiouridylase subunit 2 homolog (S. pombe); NCS2; PF0432. |
| Background | CTU2 is a 515 amino acid cytoplasmic protein that plays a role in the 2-thiolation of mcm(5)S(2)U at wobble positions of tRNA. CTU2 forms a complex with CTU1 and Urm1, and may also form a separate heterodimer with CTU1 to ligate sulfur from thiocarboxylated Urm1 onto tRNA. Existing as three alternatively spliced isoforms, the gene encoding CTU2 maps to human chromosome 16q24.3. Chromosome 16 encodes over 900 genes and comprises nearly 3% of the human genome. The GAN gene is located on chromosome 16 and, with mutation, may lead to giant axonal neuropathy, a nervous system disorder characterized by increasing malfunction with growth. The rare disorder Rubinstein-Taybi syndrome is also associated with chromosome 16, as is Crohn's disease, which is a gastrointestinal inflammatory condition. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CTU2 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-14109R-BF488) validated for, and at what dilution?
bs-14109R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CTU2 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-14109R-BF488) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Sheep, Horse.
How should bs-14109R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CTU2 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-14109R-BF488)?
KLH conjugated synthetic peptide derived from human CTU2/C16orf84
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-14109R-BF488)?
AbBy Fluor-488 on bs-14109R-BF488: excitation / emission 499nm/519nm.