CYB5R4 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-14133R-RBITC |
| Product Name | CYB5R4 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CYB5R4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 51167 |
| Swiss Prot | Q7L1T6 |
| Subcellular location | Cytoplasm |
| Synonyms | NCB5OR; cb5/cb5R; dJ676J13.1; Cytochrome b5 reductase 4; Flavohemoprotein b5/b5R; b5+b5R; N-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containing protein; CYB5R4 |
| Background | NADH-cytochrome b5 reductase involved in endoplasmic reticulum stress response pathway. Plays a critical role in protecting pancreatic beta-cells against oxidant stress, possibly by protecting the cell from excess buildup of reactive oxygen species (ROS). Reduces a variety of substrates in vitro, such as cytochrome c, feericyanide and methemoglobin. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CYB5R4 Polyclonal Antibody, RBITC Conjugated (bs-14133R-RBITC) validated for, and at what dilution?
bs-14133R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CYB5R4 Polyclonal Antibody, RBITC Conjugated (bs-14133R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Chicken.
How should bs-14133R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CYB5R4 Polyclonal Antibody, RBITC Conjugated (bs-14133R-RBITC)?
KLH conjugated synthetic peptide derived from human CYB5R4
What are the excitation and emission wavelengths of the RBITC conjugate (bs-14133R-RBITC)?
RBITC on bs-14133R-RBITC: excitation / emission 540nm/625nm.