phospho-Cyclin C (Ser275) Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-14136R-BF680 |
| Product Name | phospho-Cyclin C (Ser275) Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Horse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated Synthesised phosphopeptide derived from human Cyclin C around the phosphorylation site of Ser275 |
| Modification Site | Ser275 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 892 |
| Swiss Prot | P24863 |
| Subcellular location | Nucleus |
| Synonyms | Cyclin C (phospho S275); p-Cyclin C (phospho S275); CCNC; CCNC_HUMAN; CycC; Cyclin C; Cyclin-C; CyclinC; hSRB11; OTTHUMP00000016897; SRB11 homolog |
| Background | The protein encoded by this gene is a member of the cyclin family of proteins. The encoded protein interacts with cyclin-dependent kinase 8 and induces the phophorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The level of mRNAs for this gene peaks in the G1 phase of the cell cycle. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-Cyclin C (Ser275) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-14136R-BF680) validated for, and at what dilution?
bs-14136R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Cyclin C (Ser275) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-14136R-BF680) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Horse, Chicken.
How should bs-14136R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Cyclin C (Ser275) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-14136R-BF680)?
KLH conjugated Synthesised phosphopeptide derived from human Cyclin C around the phosphorylation site of Ser275
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-14136R-BF680)?
AbBy Fluor-680 on bs-14136R-BF680: excitation / emission 679nm/702nm.