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Cyclophilin E Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cyclophilin E Polyclonal Antibody, Unconjugated (bs-14138R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cyclophilin E Polyclonal Antibody, Unconjugated (bs-14138R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cyclophilin E Polyclonal Antibody, Unconjugated (bs-14138R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cyclophilin E Polyclonal Antibody, Unconjugated (bs-14138R) at 1:200 overnight at 4\u00b0C, DAB staining.
Bioss unconjugated antibody vial

Applications

  • ELISA
  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Horse
Overview
Catalog # bs-14138R
Product Name Cyclophilin E Polyclonal Antibody
Applications
ELISA IHC-P IHC-F IF
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Horse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Cyclophilin E
Immunogen Range 201-301/301
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 10450
Swiss Prot Q9UNP9
Subcellular location Nucleus
Synonyms Ab1-210; Cyclophilin 33; Cyclophilin E; Cyclophilin-33; CYP 33; CYP33; EC 5.2.1.8; Peptidyl prolyl cis trans isomerase E; Peptidyl-prolyl cis-trans isomerase E; Peptidylprolyl isomerase E (cyclophilin E); Peptidylprolyl isomerase E; PPIase E; PPIE; PPIE_HUMAN; Rotamase E.
Background PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Combines RNA-binding and PPIase activities. May be involved in muscle- and brain-specific processes. May be involved in pre-mRNA splicing.
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF

Frequently asked questions

Which applications is Cyclophilin E Polyclonal Antibody (bs-14138R) validated for, and at what dilution?

bs-14138R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF; ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Cyclophilin E Polyclonal Antibody (bs-14138R) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Horse.

How should bs-14138R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Cyclophilin E Polyclonal Antibody (bs-14138R)?

KLH conjugated synthetic peptide derived from human Cyclophilin E Immunogen range: 201-301/301.

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