CYP8B1 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-14165R-BF680 |
| Product Name | CYP8B1 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CYP8B1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1582 |
| Swiss Prot | Q9UNU6 |
| Subcellular location | Cytoplasm |
| Synonyms | 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase; CP8B1_HUMAN; CYP12; CYP8B1; CYPVIIIB1; Cytochrome P450 8B1; Cytochrome P450, family 8, subfamily B, polypeptide 1; Sterol 12-alpha-hydroxylase. |
| Background | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. This gene is unique among the cytochrome P450 genes in that it is intronless. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CYP8B1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-14165R-BF680) validated for, and at what dilution?
bs-14165R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CYP8B1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-14165R-BF680) react with?
Tested reactivity: Human.
How should bs-14165R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CYP8B1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-14165R-BF680)?
KLH conjugated synthetic peptide derived from human CYP8B1
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-14165R-BF680)?
AbBy Fluor-680 on bs-14165R-BF680: excitation / emission 679nm/702nm.