Help

Cytochrome p450 2J2 Polyclonal Antibody, AbBy Fluor-555 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
Overview
Catalog # bs-14181R-BF555
Product Name Cytochrome p450 2J2 Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human
Specifications
Conjugation AbBy Fluor-555
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Cytochrome p450 2J2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1573
Swiss Prot P51589
Subcellular location Cell membrane
Synonyms Arachidonic acid epoxygenase; CP2J2_HUMAN; CPJ2; CYP2J2; CYPIIJ2; Cytochrome P450 2J2; Cytochrome P450 family 2 subfamily J polypeptide 2; Cytochrome P450 subfamily IIJ (arachidonic acid epoxygenase) polypeptide 2; Cytochrome P450 subfamily IIJ polypeptide 2; Flavoprotein linked monooxygenase; Microsomal monooxygenase; OTTHUMP00000010550.
Background This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is thought to be the predominant enzyme responsible for epoxidation of endogenous arachidonic acid in cardiac tissue. Multiple transcript variants have been found for this gene. [provided by RefSeq, Jan 2016].
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Cytochrome p450 2J2 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-14181R-BF555) validated for, and at what dilution?

bs-14181R-BF555 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Cytochrome p450 2J2 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-14181R-BF555) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human.

How should bs-14181R-BF555 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Cytochrome p450 2J2 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-14181R-BF555)?

KLH conjugated synthetic peptide derived from human Cytochrome p450 2J2

What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-14181R-BF555)?

AbBy Fluor-555 on bs-14181R-BF555: excitation / emission 553nm/568nm.