BOB1 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1418R-Cy5 |
| Product Name | BOB1 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Horse, Chicken |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human BOb-1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5450 |
| Subcellular location | Nucleus |
| Synonyms | B-cell oct-binding protein 1; B-cell-specic coactivator OBF-1; POU domain class 2-associating factor 1; POU2AF1; OCT-binding factor 1; BOB-1; OCA-B. OBF1; OCA-B; OBF1_HUMAN. |
| Background | This gene encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Its ligands included a variety of aromatic hydrocarbons |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is BOB1 Polyclonal Antibody, Cy5 Conjugated (bs-1418R-Cy5) validated for, and at what dilution?
bs-1418R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does BOB1 Polyclonal Antibody, Cy5 Conjugated (bs-1418R-Cy5) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Horse, Chicken.
How should bs-1418R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise BOB1 Polyclonal Antibody, Cy5 Conjugated (bs-1418R-Cy5)?
KLH conjugated synthetic peptide derived from human BOb-1
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-1418R-Cy5)?
Cy5 on bs-1418R-Cy5: excitation / emission 625,650nm/670nm.