MC1R Polyclonal Antibody

Name: MC1R Polyclonal Antibody
Brand: Bioss
SKU: bs-1419R
Price: 375.0 USD
Availability: InStock

$Lane 1:mouse kidney lysates Lane 2: mouse brain lysates probed with Anti-MC-1R\/MC1 Receptor Polyclonal Antibody, Unconjugated (bs-1419R) at 1:200 in 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 35kD. Observed band size: 35kD.$

Lane 1:mouse kidney lysates Lane 2: mouse brain lysates probed with Anti-MC-1R\/MC1 Receptor Polyclonal Antibody, Unconjugated (bs-1419R) at 1:200 in 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 35kD. Observed band size: 35kD.

$paraformaldehyde-fixed, paraffin embedded human melanoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-MC-1R Polyclonal Antibody, Unconjugated (bs-1419R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining$

paraformaldehyde-fixed, paraffin embedded human melanoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-MC-1R Polyclonal Antibody, Unconjugated (bs-1419R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining

$293T cells probed with MC-1R\/MC1 Receptor Polyclonal Antibody, ALEXA FLUOR\u00ae 488 Conjugated (bs-1419R-A488) at 1:100 for 30 minutes compared to control cells (blue) and isotype control (orange).$

293T cells probed with MC-1R\/MC1 Receptor Polyclonal Antibody, ALEXA FLUOR\u00ae 488 Conjugated (bs-1419R-A488) at 1:100 for 30 minutes compared to control cells (blue) and isotype control (orange).

$Human 293T Cells were fixed with 2% PFA (10min at room temperature) and then permeabilized with 90% ice-cold methanol for 30min on ice. The cells were then incubated in 2% BSA + 10% goat serum to block non-specific protein-protein interactions for 15 min. Cells were stained with MC-1R\/MC1 Polyclonal Antibody, Alexa Fluor 488 Conjugated (bs-1419R-A488) at 1:100 for 30 min on ice. Primary Antibody staining (green) is compared to unstained cells (dark blue), and isotype control (orange).$

Human 293T Cells were fixed with 2% PFA (10min at room temperature) and then permeabilized with 90% ice-cold methanol for 30min on ice. The cells were then incubated in 2% BSA + 10% goat serum to block non-specific protein-protein interactions for 15 min. Cells were stained with MC-1R\/MC1 Polyclonal Antibody, Alexa Fluor 488 Conjugated (bs-1419R-A488) at 1:100 for 30 min on ice. Primary Antibody staining (green) is compared to unstained cells (dark blue), and isotype control (orange).

$Lane 1: MOLT-4 Cells; Probed with MC-1R\/MC1 Polyclonal Antibody, Unconjugated (bs-1419R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: MOLT-4 Cells; Probed with MC-1R\/MC1 Polyclonal Antibody, Unconjugated (bs-1419R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Predicted Reactivity

Mouse
Rat

Overview
Catalog #	bs-1419R-100uL
Product Name	MC1R Polyclonal Antibody
Applications	WB ELISA FCM IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse MC1R
Immunogen Range	271-317/317
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	17199
Swiss Prot	Q01727
Subcellular location	Cell membrane
Synonyms	Mshra; Melanocyte-stimulating hormone receptor; MSH-R; Melanocortin receptor 1; MC1-R; Mc1r
Background	Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200

Frequently asked questions

Which applications is MC1R Polyclonal Antibody (bs-1419R) validated for, and at what dilution?

bs-1419R is validated for WB (1:300-5000); ELISA (1:500-1000); FCM (1:20-100); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does MC1R Polyclonal Antibody (bs-1419R) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.

How should bs-1419R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise MC1R Polyclonal Antibody (bs-1419R)?

KLH conjugated synthetic peptide derived from mouse MC1R Immunogen range: 271-317/317.

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MC1R Polyclonal Antibody

Applications

Conjugation Options

Reactivity

Predicted Reactivity

BiossPM Rainbow Protein Marker (10~180 kDa)

BiossPM Rainbow Protein Marker (10~245 kDa)

BiossPM Rainbow Protein Marker (10~310 kDa)

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