DENN Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-14268R-BF555 |
| Product Name | DENN Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Sheep, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DENN |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8567 |
| Swiss Prot | Q8WXG6 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Differentially expressed in normal and neoplastic cells; FLJ35600; IG20; Insulinoma glucagonoma clone 20; KIAA0358; MADD; MADD_HUMAN; MAP kinase-activating death domain protein; Rab3 GDP/GTP exchange factor; RAB3GEP; DENN |
| Background | Tumor necrosis factor alpha (TNF-alpha) is a signaling molecule that interacts with one of two receptors on cells targeted for apoptosis. The apoptotic signal is transduced inside these cells by cytoplasmic adaptor proteins. The protein encoded by this gene is a death domain-containing adaptor protein that interacts with the death domain of TNF-alpha receptor 1 to activate mitogen-activated protein kinase (MAPK) and propagate the apoptotic signal. It is membrane-bound and expressed at a higher level in neoplastic cells than in normal cells. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DENN Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-14268R-BF555) validated for, and at what dilution?
bs-14268R-BF555 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DENN Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-14268R-BF555) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Horse.
How should bs-14268R-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DENN Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-14268R-BF555)?
KLH conjugated synthetic peptide derived from human DENN
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-14268R-BF555)?
AbBy Fluor-555 on bs-14268R-BF555: excitation / emission 553nm/568nm.